منابع مشابه
Purification and characterization of colicin D.
Colicin D-CA23, obtained by sonic treatment of mitomycin C-induced cells of Escherichia coli K-12 W1485 (colD), was purified by ammonium sulfate precipitation, gel filtration on Sephadex G200, ion-exchange chromatography on diethylaminoethyl cellulose, and isoelectrofocusing. Polyacrylamide-gel electrophoresis, sedimentation velocity analysis, and antigenic analysis indicated that the preparati...
متن کاملPurification and properties of colicin K.
Colicin K was purified from Escherichia coli K-235 (Col K) as a single protein free from polysaccharide. Its molecular weight was estimated at 70,000.
متن کاملPurification and properties of the colicin E3 receptor of Escherichia coli.
Colicin E3 receptor activity was extracted from the envelope fraction of colicin-sensitive Escherichia coli cells with Triton X-100 and EDTA, and was purified by ion exchange chromatography on DEAE-cellulose. The purified receptor fraction retained full receptor activity against both colicin E2 and E3, but it had little activity towards colicin El and almost no colicin K receptor activity. A pr...
متن کاملPurification and Properties of the Colicin E3 Receptor of ITscherichia coZi*
Colicin E3 receptor activity was extracted from the envelope fraction of colicin-sensitive Escherichia coli cells with Triton X-100 and EDTA, and was purified by ion exchange chromatography on DEAE-cellulose. The purified receptor fraction retained full receptor activity against both colicin E2 and E3, but it had little activity towards colicin El and almost no colicin K receptor activity. A pr...
متن کاملStructure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import.
Colicin Ia is a 69 kDa protein that kills susceptible Escherichia coli cells by binding to a specific receptor in the outer membrane, colicin I receptor (70 kDa), and subsequently translocating its channel forming domain across the periplasmic space, where it inserts into the inner membrane and forms a voltage-dependent ion channel. We determined crystal structures of colicin I receptor alone a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)33181-8